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CGTase, was selected from B. cereus YUPP-10 by a constructed fos-mid library. We discovered that CGTase has antimicrobial activity and induces resistance. In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - Cyclodextrin glycosyltransferase (CGTase, EC 220.127.116.11) is a carbohydrate‐active enzyme that belongs to glycosyl hydrolase family 13. CGTase can catalyse hydrolysis reactions, cleaving the linkages in the starch molecule (van der Veen et al., 2000). Publisher Summary This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase).
cgtase - Cyclodextrin glycosyltransferase - Anaerobranca gottschalkii - cgtase gene & protein UniProtKB - Q5ZEQ7 (Q5ZEQ7_9FIRM) γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are extensively used in agriculture, chemicals, cosmetics, foods and pharmaceuticals . Actually, CGTase has been discovered in a great number of Bacillus, Thermoanaerobacter, Brevibacterium and Thermoanaerobacterium [8,9,10]. Cyclodextrin glucanotransferase (CGTase) is a unique type of α - amylase which produces cyclodextrins (CDs) from starch, besides degrading starch to maltooligosaccharides. The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related α-1,4-glucans. CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. CGTases differ in the amount and types of CDs produced.
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The enzyme was purified and displayed optimum activity at 50°C and pH 6 and The enzymes from the α-amylase family all share a similar α-retaining catalytic mechanism but can have different reaction and product specificities. One family member, cyclodextrin glycosyltransferase (CGTase), has an uncommonly high transglycosylation activity and is able to form cyclodextrins. We have determined the 2.0 and 2.5 Å X-ray structures of E257A/D229A CGTase in complex with The CGTase residue has a C-alpha atom located < 6 Å from a heavy atom (i.e., an atom other than H) of an amino acid residue of the maltogenic alpha-amylase corresponding to residue 190-194 of SEQ ID NO: 17.
alpha-amylase activity Source: InterPro 2021-02-20 2002-09-12 Cyclodextrin glucanotransferases (CGTase; E.C 18.104.22.168) belonging to the glycoside hydrolase family 13 (GH 13) are widely used as catalysts in starch conversion processes (Han et al. 2014). CGTases mainly perform three different reactions in addition to hydrolysis, namely … The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis. 2014-12-15 γ-CGTase activity increased, in both control cultures (from 71.7 to 97.0%) and cultures supplemented with β-cyclodextrin (from 56.3 to 91.8%). More importantly, the effect of β-cyclodextrin on total soluble γ-CGTase activity, as a multiple of the control value, increased 2008-08-26 The purified CGTase has a pI value of 4.2. The optimum pH of 6.0 and 60 °C temperature were found to be the best for CGTase activity. Purified CGTase showed 5.18 kcal/mol activation energy (Ea).
From this complex, either a cyclization or a disproportionation reaction can …
Bacillus macerans cyclodextrin glycosyltransferase (CGTase) was used to convert dodecyl-Î²-maltoside (DDM) to dodecyl-Î²-maltooctaoside (DDMO) using Î±-cyclodextrin (Î±-CD) or starch as glycosyl donors.
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typhimurium strains TA 1535, TA 153 7, T A 98, T A 100 and E. coli WP2 uvrA with and without metabolic glucanotransferase (CGTase) its cyclization via reaction . All known CGTase produce a mixture of β, and α, γ-CD, and CGTase is classified into three kinds (βα, and , γ-CGTase) based on its product specificity .
One of the techniques uses Congo red and xylene cyanole dyes which form a clear halo only in the presence of γ-cyclodextrin (γ-CD), whereas the other uses phenolphthalein that is specific for β
Bacillus macerans cyclodextrin glycosyltransferase (CGTase) (EC 22.214.171.124) was covalently immobilised on Eupergit C and used in a packed-bed reactor to investigate the continuous production of long-carbohydrate-chain alkyl glycosides from alpha-cyclodextrin (alpha-CD) and n-dodecyl-(1,4)-beta-maltopyranoside (C(12)G(2)beta). The effects of buffer ion strength and pH, and enzyme loading on …
Information on EC 126.96.36.199 - cyclomaltodextrin glucanotransferase. cyclomaltodextrins (Schardinger dextrins) of various sizes (6,7,8, etc. glucose units) are formed …
Cyclodextrin glycosyltranferase (CGTase EC 188.8.131.52) is a bacterial enzyme that converts starch and other 1,4-linked α-glucans to cyclodextrins (closed-ring structures composed mainly of 6, 7 and 8 glucosyl units, named α, β and γ-cyclodextrin, respectively) (French, 1957; Thoma and Stewart, 1965).
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CGTase, was selected from B. cereus YUPP-10 by a constructed fos-mid library. We discovered that CGTase has antimicrobial activity and induces resistance. In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - CGTase is an important industrial enzyme that can transfer starch to glycosyl groups to form cyclodextrin (Li et al., 2014c; Qi et al., 2007).